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Antibodies

Methyl-Histone H3 (Lys27) Antibody Sampler Kit

Product Sizes
1 kit
£440.00
HAK21070-1KIT
About this Product
SKU:
HAK21070
Application:
Western Blot, IHC-Paraffin, Immunocytochemistry, ChIP
Shipping Conditions:
Blue Ice
Supplier:
Huabio
Type:
Antibody
Western blot analysis of Histone H3 (mono methyl K27) on different lysates with Rabbit anti-Histone H3 (mono methyl K27) antibody (HA722376) at 1/1;000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: SH-SY5Y cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: A549 cell lysate (20 µg/Lane) Lane 5: NIH/3T3 cell lysate (20 µg/Lane) Lane 6: PC-12 cell lysate (20 µg/Lane) Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 2% BSA/TBST for 1 hour at room temperature. The primary antibody (HA722376) at 1/1;000 dilution was used in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Histone H3 (di methyl K27) on different lysates with Rabbit anti-Histone H3 (di methyl K27) antibody (HA722486) at 1/1;000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: SH-SY5Y cell lysate (20 µg/Lane) Lane 3: HCT 116 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: MCF7 cell lysate (20 µg/Lane) Lane 6: MCF7 treated with 10μM EED226 for 72 hours cell lysate (20 µg/Lane) Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722486) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Histone H3 (tri methyl K27) on different lysates with Rabbit anti-Histone H3 (tri methyl K27) antibody (HA722231) at 1/1;000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: SH-SY5Y cell lysate (20 µg/Lane) Lane 3: HCT 116 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: C6 cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: MCF7 treated with 10μM EED226 for 72 hours cell lysate (20 µg/Lane) Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722231) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (ET1701-64) at 1/20;000 dilution and competitor's antibody at 1/5;000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: A549 cell lysate (10 µg/Lane) Lane 3: HT-29 cell lysate (10 µg/Lane) Lane 4: HEK-293 cell lysate (10 µg/Lane) Lane 5: C2C12 cell lysate (10 µg/Lane) Lane 6: L-929 cell lysate (10 µg/Lane) Lane 7: C6 cell lysate (10 µg/Lane) Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-64) at 1/20;000 dilution and competitor's antibody at 1/5;000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.