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Antibodies

Loading Control Antibody Sampler Kit

Product Sizes
1 kit
£360.00
HAK21052-1KIT
About this Product
SKU:
HAK21052
Application:
Western Blot
Shipping Conditions:
Blue Ice
Supplier:
Huabio
Type:
Antibody
Western blot analysis of beta Tubulin on different lysates with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/10;000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: 293T cell lysate (15 µg/Lane) Lane 3: MCF7 cell lysate (15 µg/Lane) Lane 4: SH-SY5Y cell lysate (15 µg/Lane) Lane 5: U-2 OS cell lysate (15 µg/Lane) Lane 6: Jurkat cell lysate (15 µg/Lane) Lane 7: Neuro-2a cell lysate (15 µg/Lane) Lane 8: NIH/3T3 cell lysate (15 µg/Lane) Lane 9: PC-12 cell lysate (15 µg/Lane) Lane 10: Mouse brain tissue lysate (20 µg/Lane) Lane 11: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-4) at 1/10;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (ET1701-64) at 1/20;000 dilution and competitor's antibody at 1/5;000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: HT-29 cell lysate Lane 4: HEK-293 cell lysate Lane 5: C2C12 cell lysate Lane 6: L-929 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-64) at 1/20;000 dilution and competitor's antibody at 1/5;000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of GAPDH on different lysates with Rabbit anti-GAPDH antibody (HA721136) at 1/20;000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: A431 cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: RAW264.7 cell lysate (20 µg/Lane) Lane 6: PC-12 cell lysate (20 µg/Lane) Lane 7: Mouse heart tissue lysate (40 µg/Lane) Lane 8: Rat heart tissue lysate (40 µg/Lane) Lane 9: Human liver tissue lysate (40 µg/Lane) Lane 10: Mouse liver tissue lysate (40 µg/Lane) Lane 11: Rat liver tissue lysate (40 µg/Lane) Lane 12: Human kidney tissue lysate (40 µg/Lane) Lane 13: Mouse kidney tissue lysate (40 µg/Lane) Lane 14: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721136) at 1/20;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of beta Actin on different lysates with Rabbit anti-beta Actin antibody (HA722023) at 1/20;000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HepG2 cell lysate (10 µg/Lane) Lane 3: MCF7 cell lysate (10 µg/Lane) Lane 4: A431 cell lysate (10 µg/Lane) Lane 5: Jurkat cell lysate (10 µg/Lane) Lane 6: HEK-293 cell lysate (10 µg/Lane) Lane 7: RAW264.7 cell lysate (10 µg/Lane) Lane 8: C2C12 cell lysate (10 µg/Lane) Lane 9: PC-12 cell lysate (10 µg/Lane) Lane 10: Mouse testis tissue lysate (10 µg/Lane) Lane 11: Mouse spleen tissue lysate (10 µg/Lane) Lane 12: Rat testis tissue lysate (10 µg/Lane) Lane 13: Rat kidney tissue lysate (10 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 3 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722023) at 1/20;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.