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Antibodies

CREB Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£425.00
HA751563-100UL
About this Product
SKU:
HA751563
Application:
Western Blot, IHC-Paraffin, IHC-Frozen, Flow Cytometry
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH15-48
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within mouse CREB aa 1-280.
Isotype:
IgG
Molecular Weight:
Predicted band size: 35 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of CREB on different lysates with Rabbit anti-CREB antibody (HA751563) at 1/50;000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: Neuro-2a cell lysate (20 µg/Lane) Lane 4: PC-12 cell lysate (20 µg/Lane) Lane 5: COS-1 cell lysate (20 µg/Lane) Lane 6: Mouse brain tissue lysate (40 µg/Lane) Predicted band size: 35 kDa Observed band size: 42 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751563) at 1/50;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of CREB on different lysates with Rabbit anti-CREB antibody (HA751563) at 1/20;000 dilution. Lane 1: HeLa-parental cell lysate Lane 2: HeLa-CREB KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 35 kDa Observed band size: 42 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751563) at 1/20;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
Application: IHC-Fr Species: Rat Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required