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Antibodies

Beta III Tubulin Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£425.00
HA751554-100UL
About this Product
SKU:
HA751554
Application:
Western Blot, IHC-Paraffin, Flow Cytometry, Immunoprecipitation
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH15-32
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within mouse Beta III Tubulin aa 401-450.
Isotype:
IgG
Molecular Weight:
Predicted band size: 50 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of Beta III Tubulin on different lysates with Rabbit anti-Beta III Tubulin antibody (HA751554) at 1/2;500 dilution. Lane 1: SH-SY5Y cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (negative) (15 µg/Lane) Lane 3: U-87 MG cell lysate (15 µg/Lane) Lane 4: Neuro-2a cell lysate (15 µg/Lane) Lane 5: NIH/3T3 cell lysate (negative) (15 µg/Lane) Lane 6: Mouse brain tissue lysate (20 µg/Lane) Lane 7: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751554) at 1/2;500 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Application: IF-Tissue Species: Mouse Site: cerebellum Sample: Paraffin-embedded section Antibody concentration: 1/400
Application: IF-Tissue Species: Rat Site: cerebellum Sample: Paraffin-embedded section Antibody concentration: 1/400
Immunocytochemistry analysis of mouse neuron cells labeling Beta III Tubulin with Rabbit anti-Beta III Tubulin antibody (HA751554) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Beta III Tubulin antibody (HA751554) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594; HA1126) was used as the secondary antibody at 1/1;000 dilution.