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Antibodies

PD-L1 Recombinant Rabbit multiclonal Antibody

Product Sizes
100μl
£689.00
HA751459-100UL
About this Product
SKU:
HA751459
Application:
Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH13-23
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within human PD-L1 aa 260-290 (Cytoplasmic).
Isotype:
IgG
Molecular Weight:
Predicted band size: 33 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies
Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (HA751459) at 1/2;000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: MCF7 cell lysate (negative) Lane 3: U-87 MG cell lysate Lane 4: HepG2 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 45-55 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751459) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (HA751459) at 1/2;000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate Lane 3: J774A.1 cell lysate Lane 4: J774A.1 treated with 1μg/mL LPS for 24 hours cell lysate Lane 5: Mouse spleen tissue lysate Lane 6: Mouse lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 45-55 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751459) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.