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Antibodies

Cystatin F Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA751261-100UL
About this Product
SKU:
HA751261
Application:
Western Blot, IHC-Frozen
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH09-00
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human Cystatin F aa 1-145.
Isotype:
IgG
Molecular Weight:
Predicted band size: 16 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of Cystatin F on different lysates with Rabbit anti-Cystatin F antibody (HA751261) at 1/2;000 dilution. Lane 1: U-937 cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (negative) (20 µg/Lane) Predicted band size: 16 kDa Observed band size: 20 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751261) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunofluorescence analysis of frozen mouse spleen tissue with Rabbit anti-Cystatin F antibody (HA751261) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature; washed with PBS; and then probed with the primary antibody (HA751261; green) at 1/100 dilution overnight at 4 ℃; washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. Nuclei were counterstained with DAPI (blue).
Immunofluorescence analysis of frozen mouse brain tissue (negative) with Rabbit anti-Cystatin F antibody (HA751261) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature; washed with PBS; and then probed with the primary antibody (HA751261; green) at 1/100 dilution overnight at 4 ℃; washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. Nuclei were counterstained with DAPI (blue).