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Antibodies

Mannose Receptor(CD206) Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA751188-100UL
About this Product
SKU:
HA751188
Application:
Western Blot, IHC-Paraffin, IHC-Frozen, Immunoprecipitation
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH07-76
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within mouse Mannose Receptor(CD206) aa 1-1;409.
Isotype:
IgG
Molecular Weight:
Predicted band size: 165 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of Mannose Receptor(CD206) on different lysates with Rabbit anti-Mannose Receptor(CD206) antibody (HA751188) at 1/2;000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 20ng/mL mIL-4 and 10ng/mL mIL-13 for 24 hours cell lysate (Macrophage M2 polarization; positive) Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate (Macrophage M1 polarization; negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 165 kDa Observed band size: 200 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751188) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Mannose Receptor(CD206) on different lysates with Rabbit anti-Mannose Receptor(CD206) antibody (HA751188) at 1/1;000 dilution. Lane 1: Mouse lung tissue lysate (20 µg/Lane) Lane 2: Mouse spleen tissue lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Rat lung tissue lysate (20 µg/Lane) Lane 5: Rat liver tissue lysate (20 µg/Lane) Predicted band size: 165 kDa Observed band size: 200 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751188) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1/1;000 Antigen retrieval: Not required
Application: IHC-Fr Species: Mouse Site: Liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required