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Antibodies

ANGPTL4 Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA751156-100UL
About this Product
SKU:
HA751156
Application:
Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH07-35
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human ANGPTL4 aa 107-406.
Isotype:
IgG
Molecular Weight:
Predicted band size: 45 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of ANGPTL4 on different lysates with Rabbit anti-ANGPTL4 antibody (HA751156) at 1/2;000 dilution. Lane 1: A549 cell lysate Lane 2: SW480 cell lysate Lane 3: THP-1 cell lysate Lane 4: HUVEC cell lysate Lane 5: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 60 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751156) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of ANGPTL4 on different lysates with Rabbit anti-ANGPTL4 antibody (HA751156) at 1/2;000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ANGPTL4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 60 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751156) at 1/2;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.