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Antibodies

MAP4K1 / HPK1 Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA751145-100UL
About this Product
SKU:
HA751145
Application:
Western Blot, IHC-Paraffin, Immunoprecipitation
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH07-24
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human MAP4K1 aa 301-500.
Isotype:
IgG
Molecular Weight:
Predicted band size: 91 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of MAP4K1 / HPK1 on different lysates with Rabbit anti-MAP4K1 / HPK1 antibody (HA751145) at 1/2;000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: Raji cell lysate (20 µg/Lane) Lane 3: Ramos cell lysate (20 µg/Lane) Lane 4: Daudi cell lysate (20 µg/Lane) Lane 5: THP-1 cell lysate (20 µg/Lane) Predicted band size: 91 kDa Observed band size: 97 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751145) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-MAP4K1 / HPK1 antibody (HA751145) at 1/2;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA751145) at 1/2;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-MAP4K1 / HPK1 antibody (HA751145) at 1/2;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA751145) at 1/2;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
MAP4K1 / HPK1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA751145 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751145 at 1/1;000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5;000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA751145 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA751145 in Jurkat cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 8 seconds; ECL: K1801