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Antibodies

p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA750894-100UL
About this Product
SKU:
HA750894
Application:
Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH03-60
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human MAPK12 aa 1-367 / 367.
Isotype:
IgG
Molecular Weight:
Predicted band size: 42 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 on different lysates with Rabbit anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 antibody (HA750894) at 1/2;000 dilution. Lane 1: 293T cell lysate (30 µg/Lane) Lane 2: HeLa cell lysate (30 µg/Lane) Lane 3: K-562 cell lysate (30 µg/Lane) Lane 4: MDA-MB-231 cell lysate (30 µg/Lane) Lane 5: COS-1 cell lysate (30 µg/Lane) Lane 6: Neuro-2a cell lysate (30 µg/Lane) Lane 7: PC-12 cell lysate (30 µg/Lane) Lane 8: Mouse skeletal muscle tissue lysate (30 µg/Lane) Lane 9: Rat skeletal muscle tissue lysate (30 µg/Lane) Lane 10: Rat heart tissue lysate (30 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750894) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 with Rabbit anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 antibody (HA750894) at 1/2;000 dilution. Lane 1: Human p38 gamma recombinant protein; 30ng/Lane Lane 2: Human p38 alpha recombinant protein; 50ng/Lane Lane 3: Human p38 delta recombinant protein; 50ng/Lane Lane 4: Human p38 beta recombinant protein; 50ng/Lane Exposure time: 43 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750894) at 1/2;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.