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Antibodies

3mC Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA750862-100UL
About this Product
SKU:
HA750862
Application:
ELISA, Dot Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH03-22
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
3-mc-OVA
Isotype:
IgG
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Immunocytochemistry analysis of U-2 OS cells treated with 4mM Methyl Methanesulfonate for 1 hour labeling 3mC with Rabbit anti-3mC antibody (HA750862) at 1/100 dilution. Cells were fixed in 70% ethyl alcohol for 5 minutes at room temperature; then subjected to acid hydrolysis using 2M HCl in TBST for 30 minutes at room temperature. Permeabilized with 0.1% Triton X-100 in PBS for 15 minutes; and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-3mC antibody (HA750862) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Dot blot analysis of 3mC on different proteins with Rabbit anti-3mC antibody (HA750862) at 1/2;000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution for 1 hour at room temperature. Lane 1: Uridine-BSA (negative) Lane 2: Cytidine-BSA (negative) Lane 3: 5-Methylcytosine-BSA (negative) Lane 4: 3-Methylcytosine-BSA (positive) Lane 5: 5-Hydroxymethylcytidine-BSA (negative) Proteins loading: 50ng; 25ng; 12.5ng; 6.25ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 1 minute.