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Antibodies

FABP4 Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA750398-100UL
About this Product
SKU:
HA750398
Application:
Western Blot, IHC-Frozen
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
JM10-99
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within human FABP4 aa 60-100.
Isotype:
IgG
Molecular Weight:
Predicted band size: 15 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C or -80[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of FABP4 on different lysates with Rabbit anti-FABP4 antibody (HA750398) at 1/5;000 dilution and competitor's antibody at 1/5;000 dilution. Lane 1: Mouse white adipose tissue lysate Lane 2: Mouse brown adipose tissue lysate Lane 3: Mouse kidney tissue lysate (negative) Lane 4: Rat white adipose tissue lysate Lane 5: Rat brown adipose tissue lysate Lane 6: Rat kidney tissue lysate (negative) Lysates/proteins at 10 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 3 minutes 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750398) at 1/5;000 dilution and competitor's antibody at 1/5;000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HAEC cells labeling FABP4 with Rabbit anti-FABP4 antibody (HA750398) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FABP4 antibody (HA750398) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594; HA1126) was used as the secondary antibody at 1/1;000 dilution.
Immunofluorescence analysis of frozen mouse adipose tissue labeling FABP4 with Rabbit anti-FABP4 antibody (HA750398). The tissues were blocked in 3% BSA for 30 minutes at room temperature; washed with PBS; and then probed with the primary antibody (HA750398; green) at 1/100 dilution overnight at 4℃; washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.