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Antibodies

TNF Receptor II Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA750386-100UL
About this Product
SKU:
HA750386
Application:
Western Blot, IHC-Paraffin, Flow Cytometry, Immunoprecipitation, IHC-Frozen
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
JM113-01
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within Human TNF Receptor II aa 426-461 / 461.
Isotype:
IgG
Molecular Weight:
Predicted band size: 48 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C or -80[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of TNF Receptor II on different lysates with Rabbit anti-TNF Receptor II antibody (HA750386) at 1/2;000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: RAW264.7 cell lysate (20 µg/Lane) Lane 5: PC-12 cell lysate (20 µg/Lane) Lane 6: Mouse spleen tissue lysate (40 µg/Lane) Predicted band size: 48 kDa Observed band size: 60 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750386) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TNF Receptor II antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA750386; 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow cytometric analysis of TNF Receptor II was done on HL-60 cells. The cells were fixed; permeabilized and stained with the primary antibody (HA750386; 1/50) (red). After incubation of the primary antibody at room temperature for an hour; the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Immunofluorescence analysis of frozen mouse hippocampus tissue labeling TNF Receptor II with Rabbit anti-TNF Receptor II antibody (HA750386). The tissues were blocked in 3% BSA for 30 minutes at room temperature; washed with PBS; and then probed with the primary antibody (ET1703-63; green) at 1/100 dilution overnight at 4℃; washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.