Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Phospho-AMPK alpha 1 (S496) Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA750300-100UL
About this Product
SKU:
HA750300
Application:
Western Blot, Immunoprecipitation, IHC-Frozen
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
SD0810
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Ser496 of Human AMPK alpha 1 aa 471-520 / 559.
Isotype:
IgG
Molecular Weight:
Predicted band size: 64 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C or -80[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of Phospho-AMPK alpha 1 (S496) on different lysates with Rabbit anti-Phospho-AMPK alpha 1 (S496) antibody (HA750300) at 1/5;000 dilution. Lane 1: SiHa cell lysate Lane 2: HUVEC cell lysate Lane 3: K-562 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750300) at 1/5;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-AMPK alpha 1(S496) on HUVEC cell lysates. Lane 1: HUVEC cells; whole cell lysate; 10ug/lane Lane 2/3: HUVEC cells treated with UV(2 hours recovery); whole cell lysates; 10ug/lane Lane 4: HUVEC cells treated with UV(2 hours recovery); then treated with 2.8ug/ul lambda-PP for 30 minutes; whole cell lysates; 10ug/lane All lanes : Anti-Phospho-AMPK alpha 1(S496) antibody (HA750300) at 1:500 dilution. Anti-GAPDH antibody (ET1601-4) at 1:10;000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200;000 dilution. Predicted band size:64 kDa Observed band size:64 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 2 minutes 14 seconds
Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Phospho-AMPK alpha 1 (S496) with Rabbit anti-Phospho-AMPK alpha 1 (S496) antibody (HA750300). The tissues were blocked in 3% BSA for 30 minutes at room temperature; washed with PBS; and then probed with the primary antibody (HA750300; green) at 1/100 dilution overnight at 4℃; washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.
Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling Phospho-AMPK alpha 1 (S496) with Rabbit anti-Phospho-AMPK alpha 1 (S496) antibody (HA750300). The tissues were blocked in 3% BSA for 30 minutes at room temperature; washed with PBS; and then probed with the primary antibody (HA750300; green) at 1/100 dilution overnight at 4℃; washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.