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Antibodies

C/EBP Beta Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA750235-100UL
About this Product
SKU:
HA750235
Application:
Western Blot, Flow Cytometry, Immunoprecipitation
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
SC55-05
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within Rat C/EBP Beta aa 248-297 / 297.
Isotype:
IgG
Molecular Weight:
Predicted band size: 36 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C or -80[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Flow cytometric analysis of C/EBP Beta was done on Hela cells. The cells were fixed; permeabilized and stained with the primary antibody (HA750235; 1/50) (red). After incubation of the primary antibody at room temperature for an hour; the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1;000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of C/EBP Beta on different lysates with Rabbit anti-C/EBP Beta antibody (HA750235) at 1/1;000 dilution. Lane 1: HeLa cell lysate Lane 2: U-87 MG cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 60 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750235) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.