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Antibodies

AMPK alpha 1 Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA750147-100UL
About this Product
SKU:
HA750147
Application:
Western Blot, Flow Cytometry, Immunoprecipitation, IHC-Frozen, IHC-Paraffin
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
SU03-48
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within Human AMPK alpha aa 501-550 / 559.
Isotype:
IgG
Molecular Weight:
Predicted band size: 64 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C or -80[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of AMPK alpha 1 on different lysates with Rabbit anti-AMPK alpha 1 antibody (HA750147) at 1/5;000 dilution and competitor's antibody at 1/2;000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: 293T cell lysate (20 µg/Lane) Lane 5: HT-29 cell lysate (20 µg/Lane) Lane 6: L-929 cell lysate (20 µg/Lane) Lane 7: C6 cell lysate (20 µg/Lane) Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 1 minute 10 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750147) at 1/5;000 dilution and competitor's antibody at 1/2;000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of AMPK alpha 1 on different lysates with Rabbit anti-AMPK alpha 1 antibody (HA750147) at 1/1;000 dilution. Lane 1: 4T1 cell lysate (20 µg/Lane) Lane 2: Neuro-2a cell lysate (20 µg/Lane) Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750147) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of AMPK α with anti-AMPK alpha 1 antibody [SU03-48] (HA750147) at 1/5;000 dilution. Lane 1: Wild-type 293T whole cell lysate (20 µg). Lane 2: AMPK α knockout 293T whole cell lysate (20 µg). Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-AMPK alpha 1 antibody (HA750147; 1/5;000) and Anti-HSP90 antibody (ET1605-56; 1/10;000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling AMPK alpha 1 with Rabbit anti-AMPK alpha 1 antibody (HA750147) at 1/250 dilution and competitor's antibody at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AMPK alpha 1 antibody (HA750147) at 1/250 dilution and competitor's antibody at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594; HA1126) was used as the secondary antibody at 1/1;000 dilution.