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Antibodies

Phospho-GSK3 (alpha + beta) (Y216 + Y279) Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA750127-100UL
About this Product
SKU:
HA750127
Application:
Western Blot, IHC-Paraffin, Immunoprecipitation, Flow Cytometry
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
SY26-05
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Tyr216 and 279 of human GSK3(alpha+beta).
Isotype:
IgG
Molecular Weight:
Predicted band size: 51 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C or -80[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of Phospho-GSK3 (alpha + beta) (Y216 + Y279) on different lysates with Rabbit anti-Phospho-GSK3 (alpha + beta) (Y216 + Y279) antibody (HA750127) at 1/5;000 dilution. Lane 1: 293T cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Rat brain tissue lysate Lane 10: 293T treated with λpp for 1 hour cell lysate Lane 11: SH-SY5Y treated with λpp for 1 hour cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 47/51 kDa Observed band size: 47/51 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750127) at 1/5;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50;000 dilution was used for 1 hour at room temperature.
ICC staining of Phospho-GSK3 (alpha + beta) (Y216 + Y279) in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750127; 1/50) for 1 hour at room temperature; washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1;000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of Phospho-GSK3 (alpha + beta) (Y216 + Y279) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750127; 1/50) for 1 hour at room temperature; washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1;000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of Phospho-GSK3 (alpha + beta) (Y216 + Y279) in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750127; 1/50) for 1 hour at room temperature; washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1;000 dilution. The nuclear counter stain is DAPI (blue).