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Antibodies

Phospho-JAK2 (Y1007 + Y1008) Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA750117-100UL
About this Product
SKU:
HA750117
Application:
Western Blot, IHC-Paraffin, Immunoprecipitation
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
SY24-03
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Tyr1007 and 1008 of human JAK2.
Isotype:
IgG
Molecular Weight:
Predicted band size: 131 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C or -80[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of Phospho-JAK2 (Y1007 + Y1008) on different lysates with Rabbit anti-Phospho-JAK2 (Y1007 + Y1008) antibody (HA750117) at 1/1;000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 1mM sodium orthovanadate for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 131 kDa Observed band size: 120 kDa Exposure time: 3 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750117) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
ICC staining of Phospho-JAK2 (Y1007 + Y1008) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750117; 1/50) for 1 hour at room temperature; washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1;000 dilution. The nuclear counter stain is DAPI (blue).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Phospho-JAK2 (Y1007 + Y1008) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA750117; 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Phospho-JAK2 (Y1007 + Y1008) antibody (HA750117) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA750117) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.