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Antibodies

NF-kB p105 / p50 Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA750060-100UL
About this Product
SKU:
HA750060
Application:
Western Blot, IHC-Paraffin, Immunocytochemistry, Flow Cytometry
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
SZ20-01
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within human NFKB1 aa $330-370.
Isotype:
IgG
Molecular Weight:
Predicted band size: 105/50 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C or -80[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of NF-kB p105 / p50 on different lysates with Rabbit anti-NF-kB p105 / p50 antibody (HA750060) at 1/5;000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si NFKB1 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 105/50 kDa Observed band size: 105/50 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750060) at 1/5;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of NF-kB p105 / p50 on different lysates with Rabbit anti-NF-kB p105 / p50 antibody (HA750060) at 1/5;000 dilution. Lane 1: THP-1 cell lysate Lane 2: Raji cell lysate Lane 3: Daudi cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 105/50 kDa Observed band size: 105/50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750060) at 1/5;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of NF-kB p105 / p50 on different lysates with Rabbit anti-NF-kB p105 / p50 antibody (HA750060) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: PC-12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 50/105 kDa Observed band size: 50/105 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750060) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NF-kB p105 / p50 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA750060; 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.