Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Mouse CCL5/RANTES Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA725047-100UL
About this Product
SKU:
HA725047
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH14-16
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within Mouse CCL5/RANTES aa 24-91 (HA211095).
Isotype:
IgG
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Sandwich ELISA analysis of mouse CCL5 matched pair antibodies Capture: HA725046; Mouse CCL5/RANTES Rabbit mAb [PSH14-15] Detector: HA725047; Mouse CCL5/RANTES Rabbit mAb [PSH14-16] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725046) diluted in carbonate/bicarbonate buffer; at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed; blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer; and incubated with serial diluted Recombinant Mouse CCL5/RANTES protein (HA211095) starting from 4;000 pg/ml to 0 pg/ml and detect antibody (HA725047; Biotin; 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CCL5 in mouse samples. Capture: HA725046; Mouse CCL5/RANTES Rabbit mAb [PSH14-15] Detector: HA725047; Mouse CCL5/RANTES Rabbit mAb [PSH14-16] Interpolated concentration of native CCL5 was measured in duplicate at different sample concentrations and interpolated from the CCL5 standard curves. Undiluted samples were 100% mouse serum. The mean CCL5 concentration was determined to be 162 pg/mL in mouse serum.