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Antibodies

ABCA7 Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£425.00
HA723974-100UL
About this Product
SKU:
HA723974
Application:
Western Blot, IHC-Paraffin
Buffer:
PBS (pH7.4); 0.1% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
PSH18-11
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within mouse ABCA7 aa 60-432.
Isotype:
IgG
Molecular Weight:
Predicted band size: 234 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of ABCA7 on different lysates with Rabbit anti-ABCA7 antibody (HA723974) at 1/5;000 dilution. Lane 1: Neuro-2a cell lysate (no heat) (20 µg/Lane) Lane 2: Mouse brain tissue lysate (no heat) (40 µg/Lane) Lane 3: Mouse cerebellum (P7) tissue lysate (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 234 kDa Observed band size: 250 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723974) at 1/5;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-ABCA7 antibody (HA723974) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA723974) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-ABCA7 antibody (HA723974) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA723974) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.