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Antibodies

Mouse EPO/Erythropoietin Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA723910-100UL
About this Product
SKU:
HA723910
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH17-23
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within Mouse EPO aa 27-192 (HA211068).
Isotype:
IgG
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Sandwich ELISA analysis of Mouse EPO/Erythropoietin matched pair antibodies Capture: HA723910; Mouse EPO/Erythropoietin Rabbit mAb [PSH17-23] Detector: HA723909; Mouse EPO/Erythropoietin Rabbit mAb [PSH17-22] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723910) diluted in carbonate/bicarbonate buffer; at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed; blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer; and incubated with serial diluted Recombinant Mouse EPO/Erythropoietin protein (HA211068) starting from 2;000 pg/ml to 0 pg/ml and detect antibody (HA723909; Biotin; 0.1 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native Erythropoietin in mouse Balb/C serum samples. Capture: HA723910; Mouse EPO/Erythropoietin Rabbit mAb [PSH17-23] Detector: HA723909; Mouse EPO/Erythropoietin Rabbit mAb [PSH17-22] The concentrations of Erythropoietin were measured in duplicates; interpolated from the Erythropoietin standard curve and corrected for sample dilution. Undiluted samples are Balb/C serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD; n=2). The mean Erythropoietin concentration was determined to be 132.7 pg/ml in Balb/C serum.
Interpolated concentrations of spiked Erythropoietin in mouse cell culture media samples. Capture: HA723910; Mouse EPO/Erythropoietin Rabbit mAb [PSH17-23] Detector: HA723909; Mouse EPO/Erythropoietin Rabbit mAb [PSH17-22] The concentrations of Erythropoietin were measured in duplicates; interpolated from the Erythropoietin standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD; n=2).