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Antibodies

Human CSF-1-R Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£449.00
HA723478H-100UL
About this Product
SKU:
HA723478H
Application:
ELISA
Buffer:
PBS (pH7.4); 0.1% BSA; 40% Glycerol.
translate.label.attr.clone:
PSH12-66
Clonality:
Monoclonal
Conjugate:
HRP-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within Human CSF-1-R aa 20-517 (HA211149).
Isotype:
IgG
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Sandwich ELISA analysis of Human CSF-1-R matched pair antibodies Capture: HA723477; Human CSF-1-R Rabbit mAb [PSH12-68] Detector: HA723474; Human CSF-1-R Rabbit mAb [PSH12-66] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723477) diluted in carbonate/bicarbonate buffer; at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed; blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer; and incubated with serial diluted Recombinant Human CSF-1-R protein (HA211149) starting from 111;000 pg/ml to 0 pg/ml and detect antibody (HA723474; HRP; 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.