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Antibodies

ENPP1 / PC1 Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£425.00
HA723427-100UL
About this Product
SKU:
HA723427
Application:
Western Blot, Immunohistochemistry
Buffer:
PBS (pH7.4); 0.1% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
PSH12-11
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human ENPP1 aa 576-925.
Isotype:
IgG
Molecular Weight:
Predicted band size: 105 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of ENPP1 / PC1 on different lysates with Rabbit anti-ENPP1 / PC1 antibody (HA723427) at 1/1;000 dilution. Lane 1: HepG2 cell lysate Lane 2: MDA-MB-231 cell lysate Lane 3: Huh7 cell lysate Lane 4: THP-1 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 105 kDa Observed band size: 140 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723427) at 1/5;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ENPP1 / PC1 antibody (HA723427) at 1/1;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA723427) at 1/1;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.