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Antibodies

6X His tag Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£449.00
HA722965-100UL
About this Product
SKU:
HA722965
Application:
Western Blot, IHC-Paraffin
Buffer:
PBS (pH7.4); 0.1% BSA; 40% Glycerol.
translate.label.attr.clone:
PSH07-10
Clonality:
Monoclonal
Conjugate:
HRP-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide immune sequence is HHHHHHHHC.
Isotype:
IgG
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of 6X His tag on different lysates with Rabbit anti-6X His tag antibody (HA722965) at 1/20;000 dilution. Lane 1: 293T transfected with His-tagged empty control cell lysate Lane 2: 293T transfected with His-tagged ACAT2 (N-terminal) cell lysate Lane 3: 293T transfected with His-tagged Histone H3.1 (C-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722965) at 1/20;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.
Immunohistochemical analysis of paraffin-embedded HeLa transfected with His-tagged ACAT2 (N-terminal) cells with Rabbit anti-6X His tag antibody (HA722965) at 1/10;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA722965) at 1/10;000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded HeLa transfected with His-tagged Histon H3.1 (C-terminal) cells with Rabbit anti-6X His tag antibody (HA722965) at 1/10;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA722965) at 1/10;000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.