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Antibodies

5-HT (Serotonin) Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£449.00
HA722915-100UL
About this Product
SKU:
HA722915
Application:
ELISA
Buffer:
PBS (pH7.4); 0.1% BSA; 40% Glycerol.
translate.label.attr.clone:
PSH06-04
Clonality:
Monoclonal
Conjugate:
HRP-conjugated
Host:
Rabbit
Immunogen:
5-HT-OVA
Isotype:
IgG
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Direct ELISA analysis of 5-HT was performed by coating wells of a 96-well plate with 50 µL per well of 5-HT-BSA diluted in carbonate/bicarbonate buffer; at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed; blocked with 1%BSA blocking buffer; and incubated with 50 µL per well of 5-HT-HRP monoclonal antibody serial diluted starting from a concentration of 4µg/mL for 45 min at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.