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Antibodies

PI3 Kinase p85 alpha Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£425.00
HA722522-100UL
About this Product
SKU:
HA722522
Application:
Western Blot
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JE04-11
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within human PI3 Kinase p85 alpha aa 675-724 / 724.
Isotype:
IgG
Molecular Weight:
Predicted band size: 84 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of PI3 Kinase p85 alpha on different lysates with Rabbit anti-PI3 Kinase p85 alpha antibody (HA722522) at 1/1;000 dilution. Lane 1: Jurkat cell lysate Lane 2: MCF7 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C6 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722522) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.