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Antibodies

Human IL-15 Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA722103-100UL
About this Product
SKU:
HA722103
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH04-20
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within Human IL-15 aa 49-162 (P40933).
Isotype:
IgG
Molecular Weight:
Predicted band size: 18.1 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Sandwich ELISA analysis of Human IL-15 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722102) diluted in carbonate/bicarbonate buffer; at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed; blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer; and incubated with serial diluted Recombinant Human IL-15 protein (HA210773) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722103) (Biotin-conjugated; 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
The concentrations of IL-15 were interpolated from the IL-15 standard curves. The mean IL-15 concentration was determined to be 65 pg/mL in HuT-102 supernatant. There was no detectable signal in THP-1 supernatant and human serum.