Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Human IL-12B p40 Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA721934-100UL
About this Product
SKU:
HA721934
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH03-04
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human IL-23A aa 20-189.
Isotype:
IgG
Molecular Weight:
Predicted band size: 20.7 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Sandwich ELISA analysis of human IL-23 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721933) diluted in carbonate/bicarbonate buffer; at a concentration of 4 µg/ml overnight at 4℃. Wells of the plate were washed; blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer; and incubated with serial diluted human IL-23 protein starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA721934)-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Conditioned media was harvested after 16 hours. IL-23 was measured in 100% unstimulated and LPS stimulated THP-1 supernatant. The concentrations of IL-23 were interpolated from the IL-23 standard curves. The mean IL-23 concentration was determined to be 1407 pg/mL in LPS stimulated THP-1 supernatant. There was no detectable signal in unstimulated supernatant.