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Antibodies

SLC12A1 / NKCC2 Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£425.00
HA721906-100UL
About this Product
SKU:
HA721906
Application:
Western Blot, IHC-Paraffin
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JE64-26
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within Human SLC12A1 / NKCC2 aa 593-813 / 1;099.
Isotype:
IgG
Molecular Weight:
Predicted band size: 121 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of SLC12A1 / NKCC2 on different lysates with Rabbit anti-SLC12A1 / NKCC2 antibody (HA721906) at 1/1;000 dilution. Lane 1: mouse kidney tissue lysate (no heat) Lane 2: mouse kidney tissue lysate Lane 3: rat kidney tissue lysate Lane 4: K-562 cell lysate (no heat) Notice: no heat means the lysate is not boiled. Tissue lysates at 30 µg/Lane. Cell lysates at 20 µg/Lane. Predicted band size: 121 kDa Observed band size: 150 kDa Exposure time: 1 minutes 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721906) at 1/1;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Fluorescence multiplex immunohistochemical analysis of mouse kidney (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-NPHS2 (ET7107-34; Red); anti-Laminin beta 1 (ET1703-14; Green); anti-Histone H3 (EM30605; Blue); anti-SLC12A1 / NKCC2 (HA721906; Cyan) and anti-CK18 (ET1603-8; Magenta) on kidney. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101; www.luminiris.cn). The section was incubated in five rounds of staining: in the order of ET7107-34 (1/1;000 dilution); ET1703-14 (1/1;000 dilution); EM30605 (1/500 dilution); HA721906 (1/3;000 dilution) and ET1603-8 (1/3;000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. Image acquisition was performed with Olympus VS200 Slide Scanner.
Fluorescence multiplex immunohistochemical analysis of mouse kidney (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-Laminin beta 1 (ET1703-14; Yellow); anti-NPHS2 (ET7107-34; Red) and anti-SLC12A1/NKCC2 (HA721906; Violet) on kidney. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101; www.luminiris.cn). The section was incubated in three rounds of staining: in the order of ET1703-14 (1/1;000 dilution); ET7107-34 (1/1;000 dilution) and HA721906 (1/3;000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-SLC12A1 / NKCC2 antibody (HA721906) at 1/20;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA721906) at 1/20;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.