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Antibodies

Human Interferon gamma Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£689.00
HA721683-100UL
About this Product
SKU:
HA721683
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH01-45
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human Interferon gamma aa 24-161.
Isotype:
IgG
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Standard curve of human IFNG matched pair antibodies: Capture: HA721683; Interferon gamma Rabbit mAb [PSH01-45] Detector: HA721685; Interferon gamma Rabbit mAb [PSH01-47] Sandwich ELISA analysis of human IFNG matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody [PSH01-45] diluted in carbonate/bicarbonate buffer; at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed; blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer; and incubated with serial diluted IFNG protein starting from 2500 pg/ml to 0 pg/ml and detect antibody [PSH01-47]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native IFN-gamma in human PBMC cell culture supernatant. Capture: HA721683; Interferon gamma Rabbit mAb [PSH01-45] Detector: HA721685; Interferon gamma Rabbit mAb [PSH01-47] PBMC cells were stimulated with 10 µg/ml PHA-M or vehicle control and incubated for 48 hours. The concentrations of IFN-gamma were interpolated from the IFN-gamma standard curves and corrected for sample dilution. Undiluted samples are PHA-M stimulated PBMC supernatant 10% and unstimulated PBMC supernatant 10%. The mean IFN-gamma concentration was determined to be 12;543 pg/ml in PHA-M stimulated PBMC supernatant and undetectable in the unstimulated PBMC supernatant.