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Antibodies

Phospho-Chk2 (T68) Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£425.00
HA721633-100UL
About this Product
SKU:
HA721633
Application:
Western Blot
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JE43-12
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Thr68 of human Chk2.
Isotype:
IgG
Molecular Weight:
Predicted band size: 61 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of Phospho-Chk2 (T68) on different lysates with Rabbit anti-Phospho-Chk2 (T68) antibody (HA721633) at 1/2;000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 20μM Etoposide for 2 hours cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 25μM Etoposide for 5 hours cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 25μM Etoposide for 5 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721633) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-Chk2 (T68) on different lysates with Rabbit anti-Phospho-Chk2 (T68) antibody (HA721633) at 1/1;000 dilution. Lane 1: C6 cell lysate Lane 2: C6 treated with 25μM Etoposide for 5 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721633) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50;000 dilution was used for 1 hour at room temperature.