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Antibodies

Liver Carboxylesterase 1 Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£425.00
HA721416-100UL
About this Product
SKU:
HA721416
Application:
Western Blot, IHC-Paraffin, Flow Cytometry
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JE33-04
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within Human Liver Carboxylesterase 1 aa 51-100 / 567.
Isotype:
IgG
Molecular Weight:
Predicted band size: 62.5 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of Liver Carboxylesterase 1 on different lysates with Rabbit anti-Liver Carboxylesterase 1 antibody (HA721416) at 1/1;000 dilution. Lane 1: U-937 cell lysate (20 µg/Lane) Lane 2: Human liver tissue lysate (10 µg/Lane) Lane 3: Human lung tissue lysate (10 µg/Lane) Predicted band size: 62.5 kDa Observed band size: 55 kDa Exposure time: 16 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721416) at 1/1;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Liver Carboxylesterase 1 on different lysates with Rabbit anti-Liver Carboxylesterase 1 antibody (HA721416) at 1/1;000 dilution. Lane 1: Mouse kidney tissue lysate Lane 2: Rat liver tissue lysate Lane 3: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 62.5 kDa Observed band size: 55 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721416) at 1/1;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Liver Carboxylesterase 1 antibody (HA721416) at 1/1;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA721416) at 1/1;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry analysis of U-937 cells labeling Liver Carboxylesterase 1 with Rabbit anti-Liver Carboxylesterase 1 antibody (HA721416) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Liver Carboxylesterase 1 antibody (HA721416) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594; HA1126) was used as the secondary antibody at 1/1;000 dilution.