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Antibodies

Dihydrofolate reductase Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£425.00
HA721394-100UL
About this Product
SKU:
HA721394
Application:
Western Blot, IHC-Paraffin
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JE31-05
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within Human Dihydrofolate reductase aa 88-187 / 187.
Isotype:
IgG
Molecular Weight:
Predicted band size: 21.5 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of Dihydrofolate reductase on different lysates with Rabbit anti-Dihydrofolate reductase antibody (HA721394) at 1/1;000 dilution. Lane 1: HCT116 cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (15 µg/Lane) Lane 3: U-2 OS cell lysate (15 µg/Lane) Lane 4: K-562 cell lysate (15 µg/Lane) Lane 5: MCF7 cell lysate (15 µg/Lane) Lane 6: HEK-293 cell lysate (15 µg/Lane) Lane 7: Jurkat cell lysate (15 µg/Lane) Lane 8: HepG2 cell lysate (15 µg/Lane) Lane 9: LO2 cell lysate (15 µg/Lane) Lane 10: Mouse liver tissue lysate (30 µg/Lane) Lane 11: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 21.5 kDa Observed band size: 21.5 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721394) at 1/1;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Dihydrofolate reductase antibody (HA721394) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA721394) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Dihydrofolate reductase antibody (HA721394) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA721394) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.