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Antibodies

Actin Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£449.00
HA721186-100UL
About this Product
SKU:
HA721186
Application:
Western Blot
Buffer:
PBS (pH7.4); 0.1% BSA; 40% Glycerol.
translate.label.attr.clone:
JJ09-29
Clonality:
Monoclonal
Conjugate:
HRP-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within Human Actin aa 45-80 / 377.
Isotype:
IgG
Molecular Weight:
Predicted band size: 42 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of Actin on different lysates with Rabbit anti-Actin antibody (HA721186) at 1/5;000 dilution. Lane 1: Hela cell lysate (10 µg/Lane) Lane 2: A431 cell lysate (10 µg/Lane) Lane 3: Jurkat cell lysate (10 µg/Lane) Lane 4: NIH/3T3 cell lysate (10 µg/Lane) Lane 5: PC-12 cell lysate (10 µg/Lane) Lane 6: Mouse brain tissue lysate (20 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721186) at 1/5;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.