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Antibodies

EpCAM Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£469.00
HA720217F-100UL
About this Product
SKU:
HA720217F
Buffer:
Supplied in phosphate-buffered solution; pH 7.2; containing 0.2% ProClean 950 and BSA.
translate.label.attr.clone:
PS01-69
Clonality:
Monoclonal
Conjugate:
iFluor™ 488
Host:
Rabbit
Immunogen:
Synthetic peptide.
Isotype:
IgG
Molecular Weight:
Predicted band size: 35 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Immunocytochemistry analysis of HT-29 cells labeling EpCAM with Rabbit anti-EpCAM antibody (HA720217F) at 1/1;000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EpCAM antibody (HA720217F; iFluor™ 488) at 1/1;000 dilution in 1% BSA in PBST overnight at 4 ℃. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594; HA1126) was used as the secondary antibody at 1/1;000 dilution.