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Antibodies

Actin Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£469.00
HA720160F-100UL
About this Product
SKU:
HA720160F
Application:
Flow Cytometry, Western Blot
Buffer:
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol; 1% BSA; 68.98% PBS
translate.label.attr.clone:
JJ09-29
Clonality:
Monoclonal
Conjugate:
iFluor™ 488
Host:
Rabbit
Immunogen:
Synthetic peptide within Human Actin aa 45-80 / 377.
Isotype:
IgG
Molecular Weight:
Predicted band size: 42 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Immunocytochemistry analysis of NIH/3T3 cells labeling Actin with Rabbit anti-Actin antibody (HA720160F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes; permeabilized with 0.1% Triton X-100 in PBS for 15 minutes; and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Actin antibody (HA720160F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2; red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594; HA1126) were used as the secondary antibody at 1/800 dilution.
Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Actin (HA720160F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature; washed with PBS. And then probed with the primary antibody Actin (HA720160F; iFluor™ 488) at 1/50 dilution overnight at 4 ℃; washed with PBS. DAPI was used as nuclear counterstain.
Flow cytometric analysis of NIH/3T3 cells labeling Actin. Cells were fixed and permeabilized. Then incubated for 30 minutes at +4℃ with Actin (HA720160F; red; 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488; green; 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).