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Antibodies

Cytokeratin 17 Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£469.00
HA720150F-100UL
About this Product
SKU:
HA720150F
Buffer:
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol; 1% BSA; 68.98% PBS
translate.label.attr.clone:
SR45-06
Clonality:
Monoclonal
Conjugate:
iFluor™ 647
Host:
Rabbit
Immunogen:
Synthetic peptide within Human Cytokeratin 17 aa 1-50 / 432.
Isotype:
IgG
Molecular Weight:
Predicted band size: 48 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Immunocytochemistry analysis of Hela cells labeling Cytokeratin 17 with Rabbit anti-Cytokeratin 17 antibody (HA720150F) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes; permeabilized with 0.1% Triton X-100 in PBS for 15 minutes; and then blocked with 2% normal goat serum for 1 hour at 37℃. Cells were then incubated with Rabbit anti-Cytokeratin 17 antibody (HA720150F; red) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2; green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488; HA1125) was used as the secondary antibody at 1/800 dilution.
Immunofluorescence analysis of paraffin-embedded human esophagus tissue labeling Cytokeratin 17 (HA720150F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature; washed with PBS. And then probed with the primary antibodies Cytokeratin 17 (HA720150F; red) at 1/50 dilution and Vimentin (EM0401; green) at 1/1;000 dilution overnight at 4 ℃; washed with PBS. iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1;000 dilution. DAPI was used as nuclear counterstain.