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Antibodies

AMSH Recombinant Rabbit monoclonal Antibody

Product Sizes
100μl
£425.00
HA720085-100UL
About this Product
SKU:
HA720085
Application:
Western Blot
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JE63-41
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within human AMSH aa 151-200/424.
Isotype:
IgG
Molecular Weight:
Predicted band size: 48 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of AMSH on different lysates with Rabbit anti-AMSH antibody (HA720085) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: 293T cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720085) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200;000 dilution was used for 1 hour at room temperature.
Western blot analysis of AMSH on different lysates with Rabbit anti-AMSH antibody (HA720085) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: SW480 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 48 kDa Observed band size: 50kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720085) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200;000 dilution was used for 1 hour at room temperature.