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Antibodies

alpha smooth muscle Actin Recombinant Chimeric Antibody Antibody

Product Sizes
100μl
£425.00
HA610362-100UL
About this Product
SKU:
HA610362
Application:
Western Blot, IHC-Paraffin, Flow Cytometry
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
SY02-64
Conjugate:
Non-conjugated
Immunogen:
Synthetic peptide within N-terminal human alpha smooth muscle Actin.
Molecular Weight:
Predicted band size: 42 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies
Western blot analysis of alpha smooth muscle Actin on different lysates with Mouse anti-alpha smooth muscle Actin antibody (HA610362) at 1/5;000 dilution. Lane 1: Saos-2 cell lysate Lane 2: A431 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse skin tissue lysate Lane 7: Rat skin tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610362) at 1/5;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Mouse anti-alpha smooth muscle Actin antibody (HA610362) at 1/80;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610362) at 1/80;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Mouse anti-alpha smooth muscle Actin antibody (HA610362) at 1/80;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610362) at 1/80;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Mouse anti-alpha smooth muscle Actin antibody (HA610362) at 1/80;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610362) at 1/80;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.