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Antibodies

PGP9.5 Recombinant Chimeric Antibody Antibody

Product Sizes
100μl
£425.00
HA610345-100UL
About this Product
SKU:
HA610345
Application:
IHC-Frozen, IHC-Paraffin, Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
JM10-59
Conjugate:
Non-conjugated
Immunogen:
Synthetic peptide within Human PGP95 aa 191-223 / 223.
Molecular Weight:
Predicted band size: 25 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies
Application: IHC-Fr Species: Rat Site: pancreas Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required
Application: IF-Tissue Species: Human Site: pancreas Sample: Paraffin-embedded section Antibody concentration: 1/500
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rat anti-PGP9.5 antibody (HA610345) at 1/2;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610345) at 1/2;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rat anti-PGP9.5 antibody (HA610345) at 1/2;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610345) at 1/2;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.