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Antibodies

GFAP Recombinant Chimeric Antibody Antibody

Product Sizes
100μg
£689.00
HA610317-100UG
About this Product
SKU:
HA610317
Application:
IHC-Frozen, Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
SA03-04
Conjugate:
Non-conjugated
Immunogen:
Synthetic peptide within Human GFAP aa 1-50 / 432.
Molecular Weight:
Predicted band size: 50 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies
Application: IHC-Fr Species: Rat Site: cerebellum Sample: Frozen section Antibody concentration: 1/1;000 (GFAP; HA610317; Rat; red); 1/500 (GAD65; HA722750; Rabbit; green) Antigen retrieval: Not required
Western blot analysis of GFAP on different lysates with Rat anti-GFAP antibody (HA610317) at 1/2;000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse liver tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610317) at 1/2;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50;000 dilution was used for 1 hour at room temperature.