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Antibodies

Myelin PLP Recombinant Chimeric Antibody Antibody

Product Sizes
100μg
£689.00
HA610303-100UG
About this Product
SKU:
HA610303
Application:
IHC-Frozen, Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH08-21
Conjugate:
Non-conjugated
Immunogen:
Recombinant protein within human Myelin PLP aa 1-233.
Molecular Weight:
Predicted band size: 30 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
Western blot analysis of Myelin PLP on different lysates with Guinea pig anti-Myelin PLP antibody (HA610303) at 1/5;000 dilution. Lane 1: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 2: Mouse liver tissue lysate (negative) (20 µg/Lane) Lane 3: Rat brain tissue lysate (no heat) (20 µg/Lane) Lane 4: Rat liver tissue lysate (negative) (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 30 kDa Observed band size: 25/20 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610303) at 1/5;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50;000 dilution was used for 1 hour at room temperature.