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Antibodies

c-Fos Recombinant Chimeric Antibody Antibody

Product Sizes
100μg
£689.00
HA610219-100UG
About this Product
SKU:
HA610219
Application:
IHC-Frozen, IHC-Paraffin, Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH05-77
Conjugate:
Non-conjugated
Immunogen:
Recombinant protein within human Protein c-Fos aa 1-380.
Isotype:
IgG1
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies
Application: IHC-Fr Species: Mouse Site: Cerebral cortex (restraint stress induced) Sample: Frozen section Antibody concentration: 1/5;000 (c-Fos; HA610219; Rat; green); 1/1;000 (Iba1; ET1705-78; Rabbit; red) Antigen retrieval: Not required Important Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer.
Application: IHC-Fr Species: Rat Site: Cerebral cortex (restraint stress induced) Sample: Frozen section Antibody concentration: 1/2;000 (c-Fos; HA610219; Rat; green); 1/1;000 (Iba1; ET1705-78; Rabbit; red) Antigen retrieval: Not required Important Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer.
Immunohistochemical analysis of paraffin-embedded mouse hippocampus (restraint stress induced) tissue with Rabbit anti-c-Fos antibody (HA610219) at 1/5;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610219) at 1/5;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse brain (restraint stress induced) tissue with Rabbit anti-c-Fos antibody (HA610219) at 1/5;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610219) at 1/5;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.