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Antibodies

ACSL3 Recombinant Mouse monoclonal Antibody

Product Sizes
100μg
£689.00
HA610091-100UG
About this Product
SKU:
HA610091
Application:
Western Blot, IHC-Paraffin
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
A8F6-R
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within human ACSL3 aa 180-380.
Isotype:
IgG1
Molecular Weight:
Predicted band size: 80 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of ACSL3 on different lysates with Mouse anti-ACSL3 antibody (HA610091) at 1/1;000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: A431 cell lysate Lane 4: 293T cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 80 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610091) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Mouse anti-ACSL3 antibody (HA610091) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610091) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.