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Antibodies

LAMP2 Recombinant Mouse monoclonal Antibody

Product Sizes
100μg
£689.00
HA610072-100UG
About this Product
SKU:
HA610072
Application:
Western Blot, IHC-Paraffin
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
D3-D8-D3-R
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein corresponding to N terminal Human LAMP2.
Isotype:
IgG1
Molecular Weight:
Predicted band size: 45 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of LAMP2 on different lysates with Mouse anti-LAMP2 antibody (HA610072) at 1/1;000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HepG2 cell lysate Lane 4: HUVEC cell lysate Lane 5: JAR cell lysate Lane 6: HEK-293 cell lysate Lane 7: THP-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 110 kDa Exposure time: 3 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610072) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of LAMP2 on different lysates with Mouse anti-LAMP2 antibody (HA610072) at 1/2;000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-LAMP2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 110 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610072) at 1/2;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-LAMP2 antibody (HA610072) at 1/5;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610072) at 1/5;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-LAMP2 antibody (HA610072) at 1/5;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610072) at 1/5;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.