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Antibodies

6X His tag (C-terminal) Recombinant Mouse monoclonal Antibody

Product Sizes
100μg
£689.00
HA610015-100UG
About this Product
SKU:
HA610015
Application:
Western Blot, Immunoprecipitation
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
8-F0-R
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Mouse
Immunogen:
C-terminal His-tagged recombinant protein; N-terminal His-tagged recombinant protein.
Isotype:
IgG2a
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of 6X His tag (C-terminal) on C-terminal His-tag fusion protein lysates with Mouse anti-6X His tag (C-terminal) antibody (HA610015) at different dilutions. Lysates/proteins at 50 ng/Lane. Observed band size: 30 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610015) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100;000 dilution was used for 1 hour at room temperature.
His-tag was immunoprecipitated in 2µg C-terminal His-tag fusion protein lysate with HA610015. Western blot was performed from the immunoprecipitate using HA610015 at 1/2;000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150;000 dilution was used for 60 mins at room temperature. Lane 1: C-terminal His-tag fusion protein lysate (input). Lane 2: HA610015 IP in C-terminal His-tag fusion protein lysate. Lane 3: Mouse IgG instead of HA610015 IP in C-terminal His-tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST.