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Antibodies

Glucose Transporter GLUT1 Recombinant Chimeric Antibody Antibody

Product Sizes
100μl
£400.00
HA601521-100UL
About this Product
SKU:
HA601521
Application:
IHC-Frozen, IHC-Paraffin, Western Blot
Buffer:
PBS (pH7.4); 0.1% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
SA0377
Conjugate:
Non-conjugated
Immunogen:
Synthetic peptide within Human GLUT1 aa 443-492 / 492.
Molecular Weight:
Predicted band size: 54 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rat anti-Glucose Transporter GLUT1 antibody (HA601521) at 1/5;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA601521) at 1/5;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rat anti-Glucose Transporter GLUT1 antibody (HA601521) at 1/2;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA601521) at 1/2;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of Glucose Transporter GLUT1 on different lysates with Rat anti-Glucose Transporter GLUT1 antibody (HA601521) at 1/50;000 dilution. Lane 1: HeLa cell lysate Lane 2: HT-29 cell lysate Lane 3: PC-12 cell lysate Lane 4: Mouse liver tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 45-60 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601521) at 1/50;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50;000 dilution was used for 1 hour at room temperature.