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Antibodies

Mouse CD45R Recombinant Rat Monoclonal Antibody

Product Sizes
100μl
£370.00
HA601385-100UL
About this Product
SKU:
HA601385
Application:
IHC-Paraffin, Flow Cytometry
Buffer:
PBS (pH7.4); 0.1% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
PSH10-80
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Rat
Isotype:
IgG1
Molecular Weight:
Predicted band size: 145 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rat anti-Mouse CD45R antibody (HA601385) at 1/1;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA601385) at 1/1;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow cytometric analysis of mouse spleen cells labeling Mouse CD45R (HA601385). Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601385; 1/1;000). After incubation of the primary antibody at +4℃ for an hour; the cells were stained with a Dylight 488 conjugate-Goat anti-Rat IgG Secondary antibody at 1/1;000 dilution for 30 minutes at +4℃.
Application: IF-Tissue Species: Mouse Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/500