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Antibodies

Alpha 1 Acid Glycoprotein Recombinant Mouse monoclonal Antibody

Product Sizes
100μl
£400.00
HA601226-100UL
About this Product
SKU:
HA601226
Application:
Western Blot
Buffer:
PBS (pH7.4); 0.1% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
A4-G2-A3-R
Clonality:
Monoclonal
Conjugate:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within Human ORM1 aa 1-201 / 201.
Isotype:
IgG1
Molecular Weight:
Predicted band size: 24 kDa
Physical State:
Liquid
Purity:
Protein A affinity purified.
Shipping Conditions:
Blue Ice
Storage Conditions:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Supplier:
Huabio
Type:
Antibodies: Monoclonal Antibody
Western blot analysis of Alpha 1 Acid Glycoprotein on different lysates with Mouse anti-Alpha 1 Acid Glycoprotein antibody (HA601226) at 1/1;000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: HCT 116 cell lysate (20 µg/Lane) Lane 4: 293T cell lysate (20 µg/Lane) Lane 5: Human liver tissue lysate (40 µg/Lane) Predicted band size: 24 kDa Observed band size: 37/45 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601226) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.